Strain bj3505
Web10 Oct 2024 · The aim of this work has been to optimize the recombinant production and further characterization of α-galactosidase of Saccharomyces cerevisiae. Results: The … Web1 Nov 2001 · The strains BJ3505 VPS33::ProtA and BJ3505 VAM3ΔN VPS33::ProtA were created by transformation of a PCR fragment containing the sequence encoding Protein A and a kanMX6 selection marker with flanking regions of the VPS33 3′-region and the downstream sequence of the terminator ( Knop et al., 1999 ).
Strain bj3505
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Web1 Sep 1999 · One strain (BJ3505) has a deletion in the gene encoding the major vacuolar proteases and therefore accumulates catalytically inactive pro-alkaline phosphatase. The … Web1 Dec 2012 · BJ3505 CBP (calmodulin-binding peptide)–Vam3 nyv1Δ was used for trans-SNARE complex isolation (Table 1). YBT1 was deleted from BJ3505, DKY6281 and …
WebThe coding sequence of truncate gp160 was amplified by polymerase chain reaction (PCR) and cloned into yeast expression vector YEpFLAG-1 to construct plasmid o Weblated from two yeast strains that express a pro-enzyme (pro-ALP, strain BJ3505) or the respective maturase (proteinase A, strain DKY6281). Upon fusion, the maturase cleaves …
Web10 Oct 2024 · S. cerevisiae strain BJ3505. Different constructions were designed to obtain the degr ee of purification necessary for enzymatic characterization and to improve the … Web13 Oct 2024 · Trans-SNARE pairing was analyzed with vacuoles prepared from DKY6281 vam3Δ, BJ3505 nyv1Δ, and BJ3505 nyv1Δ VAM3ΔN. Vacuoles (120 μg each) were mixed …
Strain BJ3505 transformed with YEp MEL1 His was grown for 96 h in 1 L YPHSM before being centrifuged (7000 rpm for 10 min, 4 °C), and the supernatant was filtered through 0.45 µm nitrocellulose membrane filters (Millipore) before being dialysed and concentrated by tangential filtration (TFF, Millipore) … See more To select the best secretion and purification system for the ScAGal protein, different variants of the MEL1 gene were expressed in strain … See more Cell-free BJ3505/YEpMEL1His culture medium was concentrated and partially purified by ultrafiltration. The extracellular protein was then … See more ScAGal not only has strong resistance to treatment with all the proteases tested, but also a higher enzymatic activity in their presence (Fig. 3a). … See more In order to assess the best method of conserving the enzyme, a stability study was carried out in cold and environmental conditions (Additional … See more
Web1 Aug 2000 · Vacuole Isolation. Vacuoles were isolated from yeast strains BJ3505 and DKY6281 as described ().Large-scale frozen vacuole preparations of BJ3505 vacuoles were used for studies of glutathione S-transferase (GST)-Ypt7p association and for immunoprecipitation experiments. Spheroplast pellets were resuspended in an equal … lockheed t33 motoresWeb1 Sep 2016 · To determine the localization of Vtc5, the protein was genomically tagged with yeGFP at its C terminus in protease-deficient strains (BJ3505) and protease-proficient strains (DKY6281). Vtc5-yeGFP yielded a relatively weak signal around the vacuole rim in BJ3505 cells. No fluorescence signal was detected in other parts of the cell. lockheed t-33 shooting star for saleWeb15 Aug 2000 · Frozen BJ3505 vacuoles (300 μg) were diluted to 1.5 ml with PS buffer (see Materials and Methods) and sedimented (13,000 × g, 10 min, 4°C). Vacuole pellets were … india today best colleges 2021WebUsing different Rhizopus sp. strains as fermentation agents, this paper describes a global strategy to identify interactions between cultivation parameters (pH 4.75–7.75, 7.5–82.5 g/l glucose ... india today bollywood newsWeb20 Sep 2011 · Vacuoles were isolated from two yeast strains expressing either pro-alkaline phosphatase (pro-ALP; strains BJ3505 or BY4741) or the respective maturase (Pep4p, strain DKY6281). Upon fusion, the maturase cleaves and activates pro-ALP, the activity of which is then measured. We deleted proteolipids in these strain backgrounds and complemented ... india today bookWeb1 Mar 2000 · A BJ3505 wild-type culture was processed for vacuole purification . An aliquot of spheroplasted yeast cells was lysed by boiling in SDS-PAGE loading buffer, and the … lockheed t2v-1 „seastar“WebTheYeastATP-bindingCassette(ABC)TransporterYcf1p EnhancestheRecruitmentoftheSolubleSNAREVam7pto VacuolesforEfficientMembraneFusion* Receivedforpublication,November29 ... india today blogs