Micro bca reagent b
WebReagent A: 1 gm sodium bicinchoninate (BCA), 2 gm sodium carbonate, 0.16 gm sodium tartrate, 0.4 gm NaOH, and 0.95 gm sodium bicarbonate, brought to 100 ml with distilled … WebThe Thermo Academical Pierce Micro BCA Protein Assay Mounting is a feature 3-component version of our popular BCA Reagents, optimized to take (A562nm) entire albumin engrossment out dilute protein solutions (0.5 to 20 micrograms/mL).Features
Micro bca reagent b
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Web2. Prepare WR by mixing 50 parts Rapid Gold BCA Reagent A with 1 part Rapid Gold BCA Reagent B (50:1, Reagent A:B). For the above example, combine 10 mL of Reagent A with 0.2 mL of Reagent B. Note: When Rapid Gold BCA Reagent B is first added to Rapid Gold BCA Reagent A, a pale blue precipitate may be observed, but, upon WebDescription. Thermo Scientific™ Pierce Micro BCA Protein Assay uses a concentrated form of the BCA Reagents to accurately measure dilute protein solutions (0.5 to 20µg/mL). The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples.
WebBCA Protein Assay Protocol A Solutions and Reagents Kit Contents: 1. BCA Reagent A: 250 ml (sodium carbonate, sodium bicarbonate, bicinchoninic acid, and sodium tartarate in 0.1 M sodium hydroxide). 2. BCA Reagent B: 25 ml (4% cupric sulfate). 3. Compatibility Reagent: 9.3 mg × 48 microtubes. 4. Reconstitution Buffer: 15 ml. 5. WebThe Micro BCA Protein Assay Kit is a ready to use, detergent-compatible, Western blot related, total protein analysis reagent used for the quick determination of total protein concentration of dilute protein solutions by measuring absorbance at 562 nm and comparing to a protein standard absorbance vs. concentration curve.Accurately …
WebWe are the world leader in non-invasive fecal immunochemical testing technology and bladder cancer monitoring. Combined with an experienced team of field representatives … WebJan 27, 2024 · The Pierce BCA assay recommends using up to 25 microliters of unknown sample with 200 microliters of working reagent. When I get the spec concentration for example 1000microgram / milliliter,...
WebPierce® BCA Reagents Pierce® BCA Protein Assay Reagent B; Pierce® Micro BCA Reagent C; BCA Reagent B Liquid. CHEMTREC: 800.424.9300 Outside US: 703.527.3887 SAFETY …
WebThe Micro BCA Protein Assay Kit is a ready to use, detergent-compatible, Western blot related, total protein analysis reagent used for the quick determination of total protein … how to cube root in pythonWebApr 15, 2024 · The peptide reagent reported herein is a practical tool for investigating the mechanisms of disulfide-coupled folding of mid-size proteins. Next Article in Journal. S … the middle kingdom rideWebThis kit is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu 1+ ), which is generated by reduction of cupric cation (Cu 2+) by the protein in alkaline conditions. The assay is linear over a wide range of protein concentrations (25-2000 μg/mL). how to cube root in excelWebThe Bicinchoninic Acid (BCA) Protein Assay is suitable for quantifying protein solutions in 1ml assays or in micro-wells. Features Sensitive colorimetric assay Linear range of 20-2,000µg/ml Compatible with wide … how to cube red potatoesWebUrinalysis using a dipstick reagent strip is an effective screening tool to assess the health status of an individual and detect some diseases and infections. It is impor-tant that … how to cube root on desmosWebThe bicinchoninic acid assay ( BCA assay ), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, [1] now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL), similar to Lowry protein assay, Bradford protein … the middle kingdom ancient egyptWebDescription. Thermo Scientific™ Pierce Micro BCA Protein Assay uses a concentrated form of the BCA Reagents to accurately measure dilute protein solutions (0.5 to 20µg/mL). The … how to cube root a number