Hoechst stain apoptosis
NettetHoechst 33342 and LysoTracker staining. Apoptotic nuclear changes were assessed with Hoechst 33342 (Sigma-Aldrich Co.). ... (10 µM) for 8 hours also showed apoptotic nuclear chromatin condensation by confocal microscopy with Hoechst 33342 staining . Figure 2 Glucose deprivation enhances the apoptosis of HeLa cell induced by S1. NettetWe explored the specificity of AO staining while studying nuclear apoptosis in Tetrahymena. Using AO alone or together with the vital nuclear stain Hoechst 33342 (HO), we find that lysosomes are generally clustered around the degenerating nucleus and that such nuclei are stained an orange-red color, like lysosomes.
Hoechst stain apoptosis
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NettetHoechst 33,258 staining was used to evaluate cell apoptosis. HepG2 cells (10 5 /dish) were seeded in glass bottom dishes for 24 h and treated with SSPH I and U0126 for 24 h. Cells were then washed twice with PBS and then stained with Hoechst 33,258 (10 μg/mL in PBS) at 37 °C for 15 min in dark, and observed under a fluorescent inverted … NettetNuclear staining methods DAPI and Hoechst are both minor groove binders intercalating the AT regions. In case of apoptosis the cells may show ssDNA strand breaks as well …
Nettet18. jun. 2016 · Furthermore, compound 1 dose-dependently induced A2780 cells apoptosis as evidenced by Hoechst 33342 staining, Annexin V positive cells, ... compound 1 dose-dependently induced A2780 cells apoptosis as evidenced by Hoechst 33342 staining, Annexin V positive cells, the up-regulated cleaved-PARP and the … Nettet2. nov. 2012 · Hoechst 33342 is a blue fluorescing dye that stains chromatin DNA. The red fluorescing dye propidium iodide is only permeable to dead cells and cannot enter the intact plasma membrane of living cells.
NettetHoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These bis-benzimides were originally developed by Hoechst AG, which numbered all their … Nettet3. okt. 2016 · The TUNEL assay is both fast and sensitive. Here, we describe a protocol in which cells are treated with TUNEL reagent and counterstained with Hoechst 33342. In contrast to TUNEL, which only stains apoptotic cells, Hoechst 33342 stains the DNA of …
NettetDescription Description The GenScript Double Stain Apoptosis Detection Kit (Hoechst 33342/PI) provides a rapid and convenient assay for apoptosis based upon …
NettetThe Vybrant Apoptosis Assay Kit #5, or the Chromatin Condensation/Dead Cell Apoptosis Kit with Hoechst 33342 and PI for Flow Cytometry, provides a rapid and … george anton actorNettetHoechst 33342 binds all DNA therefore staining all nuclear material, allowing identification of apoptotic nuclei, but the analysis is laborious. Combining the two fluorescent labels allows the initial recognition of apoptotic cells using the TUNEL technique then, by simply changing the filter, the TUNEL positive nuclei can be … christchurchtemple.orgNettetHoechst 33258 staining and flow cytometry for cell-apoptosis assays. Cells were stained with Hoechst 33258 for apoptotic cells. Cells were washed by PBS and stained with Hoechst 33258 (5 μg/mL) for 30 minutes at room temperature. Apoptotic cells were counted under fluorescence microscopy. christchurch templeNettet15. mai 2014 · Apoptosis, a genetically programmed cellular event leads to biochemical and morphological changes in cells. Alterations in DNA caused by several factors affect nucleus and ultimately the entire cell leading to compromised function of … christchurch television stationsNettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient—provided as an easy-to … george antony mississaugaNettet11. jun. 2024 · Nuclei stained with Hoechst dye appear brighter when cells undergo apoptosis and fade when undergoing necrosis. The percentage of brightly stained nuclei is obtained using a high-content fluorescence microscopy platform and used to calculate an apoptosis index, indicating changes in apoptosis between test and control … george a. ohl jr. trustNettetIn this study we demonstrate that the common live-cell DNA stain Hoechst 33342 can cause apoptosis under CTLM conditions. Although toxicity is evident at long times in the absence of imaging at high dye concentrations, phototoxicity from repeated excitation of the dye in the imaging process is dominant. christchurch tennis club