Cholesterylation of smoothened
WebDec 16, 2024 · SMO cholesterylation is a calcium-accelerated autoprocessing reaction, and STIM1-ORAI1-mediated store-operated calcium entry promotes cholesterylation … WebNational Center for Biotechnology Information
Cholesterylation of smoothened
Did you know?
WebDec 1, 2024 · Cholesterol modification, or cholesterylation of SMO is a recently defined mechanism to activate SMO. Cholesterol covalently conjugates to the side chain … WebDec 1, 2024 · Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate 2024, Cell Research Smoothened/AMP …
WebAug 1, 2024 · Cholesterol binds and then covalently links to the D95 residue of cysteine-rich domain (CRD) of human SMO. The cholesterylation of CRD is critical for SMO … http://labsdev.utsouthwestern.edu/jiang-jin-lab/publications
WebCholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate Article Feb 2024 Hedgehog (Hh) is a morphogen that binds to its receptor... WebFeb 1, 2024 · The G-protein coupled receptor-like protein Smoothened (SMO) is one of the major members in Hh pathway. Covalent modification of cholesterol on the 95th …
WebCholesterol modification (or cholesterylation) is a rare but important posttranslational lipid modification of proteins. So far, two proteins, namely Hedgehog and Smoothened, have …
pCMV-FLAG-SMO was generated by inserting human Smo cDNA into pcDNA3, followed by inserting a 3× FLAG epitope-coding DNA after the signal peptide of SMO. The sequence after 1–192 amino acids (a.a.) was truncated to generate pCMV-FLAG-CRD. pCMV-GCaMP6s-SMO-mCherry-FLAG was generated as … See more The following antibodies were used in this study: mouse mAb anti-FLAG, clone M2 (F3165, Sigma), mouse mAb anti-acetyl Tubulin, clone 6-11B-1 (T6793, Sigma), mouse mAb anti-SMO, clone E5 (Sc-166685, Santa … See more HEK-293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific) plus 10% fetal bovine … See more Cholesterol, thapsigargin, CuSO4and sodium ascorbate were from Sigma. Methyl-β-cyclodextrin and hydroxypropyl-β-cyclodextrin were from CTD. Inc. SAG, dantrolene sodium and GANT61 were from … See more Molecular docking studies were carried out using Molecular Operating Environment (MOE 2015.10, Chemical Computing Group, Canada) … See more townhomes for rent lutherville mdWebRegulation of Smoothened trafficking and abundance in Hedgehog signaling. Frontiers in Cell and Developmental Biology 10:847844. doi: 10.3389/fcell.2024.847844. Yuhong Han and Jin Jiang. 2024. Revealing the secret behind Smoothened Cholesterylation. Cell Research 32(4):327-328. doi: 10.1038/s41422-022-00636-8. townhomes for rent mableton gaWebCholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate Citing article ... Ubiquitin specific peptidase 20 (USP20) is a member of the... townhomes for rent magnolia txWebSMO cholesterylation is a calcium-accelerated autoprocessing reaction, and STIM1-ORAI1-mediated store-operated calcium entry promotes cholesterylation and activation of endosome-localized SMO. However, it is unknown whether the Hh-PTCH1 interplay regulates the activity of the endoplasmic reticulum (ER)-localized SMO. townhomes for rent macon gatownhomes for rent mahtomedi mnWebJun 1, 2024 · The G protein-coupled receptor (GPCR)-like protein Smoothened (SMO) is the central signal transducer in the Hh pathway. Cholesterol binds and then covalently links to the D95 residue of... townhomes for rent maplewoodWebHedgehog (Hh) is a morphogen that binds to its receptor Patched 1 and activates Smoothened (SMO), thereby governing embryonic development and postnatal tissue … townhomes for rent maplewood mn